SYNTHESIS OF HIPPURIC ACID IN LIVER HOMOGENATE

Colorimetric determination of hippuric acid in urine and liver homogenate.

To a dried extract Containing hippuric acid (HA), 1.0 mL of acetic anhydride and 2.0 mL of 0.5% p-dlmethyiamlnobenzaldehyde (DAB) solution In pyridine were added, and the solution was kept at 40 O C for 1 h after thorough mlxing. The absorbance was then determlned at 458 nm agalnst a blank containing acetic anhydride, DAB, and pyridine. This method was in good agreement with Beer’s law within 1...

The Role of the Liver in Hippuric Acid Synthesis.

Studies of Liver Function. Benzoate Administration and Hippuric Acid Synthesis

Much work has been done in an effort to understand the complex function of the liver and considerable information of value has been obtained. It is fair to say, however, that the estimation of functional capacity of the liver cannot as yet be made with any convincing accuracy. We have reason to believe that the liver has a very large factor of safety-that it can tolerate an extensive injury and...

Fatty acid synthesis from acetate by human liver homogenate fractions.

Evidence obtained during the last few years has shown that fatty acid synthesis in a variety of mammalian tissues (1-4) as well as in yeast (5, 6) and bacteria (7, 8) is not simply the reverse of fatty acid oxidation. This is borne out by the finding that oxidative processes seem to be confined to the mitochondrial portion of the cell, whereas fatty acid synthesis apparently occurs in extramito...

Observations on the Interpretation of the Hippuric Acid Synthesis Test of Liver Function